Spectrophotometry Lab Simulator

Simultaneous Determination of Two Dyes in Solution

Measure an unknown mixture of the two dyes at both wavelengths and determine the concentration of each dye using the fitted calibration curves.

Step 3 Data

Fitted Calibration Curves

These fitted lines will be used to determine the concentrations of the unknown dye mixture.

Red Dye at Red Wavelength

Pending

Red Dye at Green Wavelength

Pending

Green Dye at Red Wavelength

Pending

Green Dye at Green Wavelength

Pending

Step 4

Measure the Unknown Mixture

Generate an unknown mixture, measure it at both wavelengths, and use the fitted curves to determine the concentration of each dye.

Mixture Controls

Create a random mixture and then measure it with the red and green filters.

Unknown Mixture

No mixture generated

Generate an unknown mixture to begin the mixture-analysis step.

Calculated Results

Enter the concentrations you calculate from the measured absorbances.

Measured Mixture Data

Record both wavelengths before submitting your calculated concentrations.

Wavelength Dark Current (V) 100%T (V) Raw Signal (V) %T Absorbance
Red Filter Pending Pending Pending Pending Pending
Green Filter Pending Pending Pending Pending Pending

Step 4 Status

Generate a mixture and measure it at both wavelengths before submitting your calculated concentrations.