Spectrophotometry Lab Simulator

Simultaneous Determination of Two Dyes in Solution

Use the baseline signals and measured calibration points to assemble the four calibration curves and fit each selected dataset to a line.

Step 1 Data

Baseline Signals

These baseline values are shown here so the curve-fitting step can reference them.

Wavelength Dark Current (V) 100%T (V)
Red Filter Pending Pending
Green Filter Pending Pending

Step 2 Data

Calibration Measurements

Select calibration measurements from the completed dataset and add them to the active curve if they match the chosen dye and wavelength.

Add Dye Wavelength Concentration Raw Signal (V) %T Absorbance
No calibration measurements have been saved yet.

Step 3

Create and Fit Calibration Curves

Choose one of the four dye and wavelength combinations, add selected points to the graph, and then fit the data to a line.

Curve Controls

Select which calibration curve you are building, then add measurements to it.

Calibration Curve

Selected Curve

Red Dye at Red Wavelength

Select matching calibration points from the table above, then add them to this curve.

Linear Fit

Add at least two points to the active curve and then use Fit Data to calculate the slope and intercept.

Curve Plot

The scatter plot updates for the currently selected calibration curve.

Points in Active Curve

0

Fit Status

Not Fit

Slope

Pending

Intercept

Pending