Spectrophotometry Lab Simulator

Simultaneous Determination of Two Dyes in Solution

Use the saved Step 1 baseline values to prepare diluted dye samples and collect the concentration-absorbance measurements that will feed the calibration curves.

Step 1 Data

Baseline Signals

These values were collected in Step 1 and are carried into the calibration page.

Wavelength Dark Current (V) 100%T (V)
Red Filter Pending Pending
Green Filter Pending Pending

Step 2

Collect Calibration Measurements

Prepare diluted samples, measure absorbance, and collect at least five points for each dye at each wavelength.

Calibration Data Table

Each measurement is appended below with the baseline values shown beside it.

Delete Dye Wavelength Dark Current (V) 100%T (V) Dye Volume Water Volume Concentration Raw Signal (V) %T Absorbance
-- Baseline Red Filter Pending Pending -- -- -- -- -- --
-- Baseline Green Filter Pending Pending -- -- -- -- -- --
No calibration measurements recorded yet.

Sample Preparation

Select a dye, choose the dilution, and prepare the sample before measuring.

Dye Selection
Measurement Wavelength

Current Sample

No sample prepared

Prepare a diluted sample to display its concentration before measuring.

Calibration Progress

Each combination needs at least five concentration-absorbance points.

Red Dye at Red Filter

0 / 5

Red Dye at Green Filter

0 / 5

Green Dye at Red Filter

0 / 5

Green Dye at Green Filter

0 / 5

Calibration Status

Step 1 must be completed before calibration measurements can be recorded.